Anti-Glypican 6 (Glypiated Proteoglycan 6)

Glypican 6 (glypiated proteoglycan 6) belongs to the family of membrane heparin sulfate proteoglycans that are linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. The glucosaminoglycans (GAGs) attachment sites of glypican family proteins are localized close to the cell membrane in the C-terminal region. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Mature human and mouse glypican share 98% amino acid sequence identity. Glypican 6 is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Applications: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using HepG2 human hepatocellular carcinoma cell line, Western Blot: 1ug/ml using recombinant human Glypican 6, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.