Anti-EGF-Receptor (Epidermal Growth Factor Recptor, erbB, EGFR), clone 3F155

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1,-2,-3-4 or HER-1,-2,-3-4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein A., Western Blot: -4ug/ml for 2hrs at RT, Optimal dilutions to be determined by the researcher. Positive Control: A431 cells, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.