Anti-EGF-Receptor (Epidermal Growth Factor Recptor, erbB, EGFR), clone 3F154

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1,-2,-3-4 or HER-1,-2,-3-4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml for 2hrs at RT, Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G, Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C. , Optimal dilutions to be determined by the researcher. Positive Control: A431 cells. Skin, placenta or squamous cell carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.