Anti-Cytokeratin 19 (Keratin, Type I Cytoskeletal 19, KRT-19, CK19, Keratin-19)

Cytokeratin 19 (Keratin, type I cytoskeletal 19, also KRT-19, CK19 and Keratin-19) is a 40-45kD, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52kD class II KRT-8 (plus KRT-5 and -7) to form 8-10nm filaments in epidermal stem cells, secretory gland (sweat, mammary, bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa1-79) and a subsequent "rod" region (aa80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa2-400) shares 82% aa sequence identity with mouse Cytokeratin 19. Applications: Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml, Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.