Anti-Cytochrome P450 1A1, 1A2 (CYP1A1, CYP1A2)

Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs). Both CYPs and FMOs are localized in the endoplasmic reticulum. Collectively, these enzymes catalyze the oxidation of a very broad range of substrates. Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:50 dilution will yield a positive result with 0.2ug of recombinant human cytochrome P450 1A1 and/or 1A2 using HRP-conjugated rabbit antigoat antibodies according to the manufacturer's instructions., Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.