Anti-Complement C3a Receptor (C3aR)

Complement Component 3a receptor (C3a R) is an ~90kD G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% aa identity with mouse and rat C3a R, respectively. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in a reaction <200ul., Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with human C3a R transfected NS0 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.