Anti-Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Pr

C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Binds both C3c and C3 so it is not suitable for all ELISA applications., Immunohistochemistry: Frozen sections, Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Kidney from post streptococal glomerulonephritis patients, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.