Anti-CLCN3

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011], Protein function: [Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874). [The UniProt Consortium] Recommended dilutions: WB 1:500-2000. Cellular localization: [Isoform 1]: Early endosome membrane , Multi-pass membrane protein . Late endosome membrane , Multi-pass membrane protein . Lysosome membrane , Multi-pass membrane protein . Cell membrane , Multi-pass membrane protein . Isoform 1 is localized mainly in late endosomes. ., [Isoform 2]: Golgi apparatus membrane , Multi-pass membrane protein . Cell projection, ruffle membrane , Multi-pass membrane protein . Isoform 2 is mainly enriched in the Golgi (PubMed:12471024). Colocalizes with SLC9A3R1/EBP50 in membrane ruffles (PubMed:11967229). .