Anti-CD95L (FAS Ligand)

Applications:, Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: >0.5ug/ml tDetect hFasL by direct ELISA (using 100ul/well antibody solution). Allows the detection of 0.2-0.4ng/well of recombinant hFasL. , Western Blot: 0.1-0.2ug/ml Detection limit for recombinant hFasL is 1.5-3ng/lane, under either reducing or non-reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.