Anti-CD8a (Ly2, T cell Co-receptor, Leu2, T8), FITC conjugated, clone 5K7

In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8alpha/Lyt-2) and a beta chain (CD8beta/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8, Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (acetone-fixed frozen sections), Immunofluorescence, Immunoprecipitation, In vivo and in vitro depletion of CD8a+ cells 1, 4, 5 and Fractionation of CD8a+ T cells 10. Other applications not tested. Recommended Dilution: Flow Cytometry: < 2ug/10e6 cells, Immunofluorescence: 1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.