Anti-CD7 (Cluster of Differentiation Antigen 7, GP40, LEU-9, TP41)

CD7 (Cluster of Differentiation Antigen 7, also Leu-9, TP41 and GP40) is a 40-44kD member of the Ig­superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34+ myeloid and lymphoid progenitor cells, memory CLA­ CD45RA+ T cells, and CD56+ IFN­gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context­dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155aa extracellular region (aa26-180) that shows one V­type Ig­like domain (aa26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79aa substitution for aa133­240. Over aa26-180, human CD7 shares only 43% aa sequence identity with mouse CD7. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.