Anti-CD54 (Intercellular Adhesion Molecule, ICAM-1)

Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Capture, Flow Cytometry: 5ug/ml (80ul), Immunohistochemistry: Fixed cells or tissues, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2409-10A at 5ug/ml. , 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. , 3. Cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.