Anti-CD43 (T Cell Marker, Leukosialin, Sialophorin, or Leukocyte Sialoglycoprotein)

Human CD43 is a heavily O-glycosylated and sialylated transmembrane protein that is mainly found on T cells, but not on B cells. Applications: Suitable for use in Flow Cytometry. Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (80ul), Immunohistochemistry: Fix cells or tissues, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Jurkat cells/tube were incubated 45 minutes on ice with 80ul of C2397-26A at 10ug/ml. , 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. , 3. Cells stained positive with a mean shift of 1.71 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.