Anti-CD31 (PECAM-1, Platelet gpIIa Molecule) (FITC)

CD31 is a member of the immunoglobulin superfamily. This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. CD31 is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express CD31. The reactivity of the monoclonal antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130kD molecule present on the membrane of , endothelial cell presents on all mouse blood vessels both in normal, inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human CD31. Applications: Suitable for use in Flow Cytometry, Immunoassay, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.