Anti-CD30 (Ki-1 antigen, Hodgkin & Reed-Sternberg Cell Marker), clone 3F83

CD30, a single chain glycoprotein, is synthesized as a 90kD precursor which is processed in the Golg.i complex into a membrane-bound phosphorylated mature 105/120kD glycoprotein. The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin's disease, by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varying proportion of activated T and B cells. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45). MW of Antigen: 120kD (mature), 95kD (precursor) , Applications: Suitable for use in Immunohistochemistry (paraffin). Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen and formalin/paraffin): 1:40-1:80. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Note that 1mM EDTA, pH 8.0 is better than 10mM citrate buffer, pH 6.0 for unmasking the epitope. Optimal dilutions to be determined by the researcher. , Positive Control: Hodgkin's lymphoma , Cellular Localization: Cell membrane , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.