Anti-CD3, epsilon (Early T Cell Marker), APC conjugated, clone 5K2

CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. It can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized antibody can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by this antibody may result in apoptotic cell death (1-6). Applications:  Identification and enumeration of CD3+ cells by flow cytometry 1, 2,  Immunoprecipitation 1,  Western blotting 7,  In vitro depletion of CD3+ cells 2,  In vitro activation of T cells 2-4,  Immunohistochemistry 8, Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.