Anti-CD1c (Biotin)

The human CD1 genetic locus consists of five closely related genes. Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells. CD1 molecules have a specialized lipid antigen-presenting function and it is unlikely that they present conventional peptide antigens. CD1 may also be involved in costimulation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Molt-4 cells per tube were washed and incubated 45 minutes on ice with 80ul C2252-03G at , 5ug/ml. , 2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-Phycoerythrin (S7973-88P), after which they were washed three times, fixed and analyzed by FACS., 3. Cells stained positive with a mean shift of 1.82 log 10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. , 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5ug/ml anti-CD1c antibody. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.