Anti-CD163 (Hemoglobin/Haptoglobin Complex Receptor, M130 antigen, Ber-Mac3, Ki-M8, SM4) (Biotin)

CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CD163. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.