Anti-CARD12 (IPAF, Ice Protease Activating Factor, CLAN)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF-kB signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family (1). IPAF (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner (2,3). IPAF and caspase-1 are thought to interact with each other through the association of the IPAF amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml, Human peripheral blood leukocyte (PBL) cell lysate can be used as positive control and a 110kD band can be detected. Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.