Anti-Cadherin E (Cadherin-1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Cadherins are a multifunctional family of Ca 2+ -dependent, transmembrane glycoproteins, which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (reviewed in ref 1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci?c fashion (2) . Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml , Immunoprecipitation: 2-5ug , Immunohistochemistry (formalin-fixed., paraffin embedded sections require a Heat Induced Epitope Retrieval (HIER) step prior to staining): 2-10ug/ml , , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.