Anti-Cadherin E (Cadherin-1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of teh best characterized cadherins is E-cadherin. It is a 120kD transmembrane glycoprotein consisting of an 80kD extracellular and a 40kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherins are responsible for calcium binding which allows for homophilic interactions with other E-cadherin molecules on the same cell and neighboring cells. E-cadherin can interact heterophilically with integrin aeb7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins. Establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated. This is consistent with its function of an invasion suppressor in normal epithelia. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry, and Western Blot, Inhibition effect on cell-cell adhesion. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50-1:100. Use ABC as detection method. Note: Use PBS, 0.1mM CaCl2 and 0.1mM MgCl2, Immunocytochemistry: Methanol fixation, Flow Cytometry: 1:50-1:100, Western Blot: 1:50-1:500, Optimum dilutions to be determined by researcher. Hybridoma: NSO mouse myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.