Anti-Bcl-10 (B Cell Leukemia 10, CIPER, mE10, CARMEN, CLAP)

Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase 9, and caspase 2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP (1-6). Bcl 10 is a cellular homolog of the equine Herpes virus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-kB activation. Bcl 10 interacts with caspase 9 and enhances pro-caspase-9 processing and induces apoptosis through caspase 9 activation. Bcl 10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy . Bcl 10 is expressed in many human and murine tissues and cell lines. Bcl 10 is identi?ed through its direct involvement in t(1,14)(p22,q32) of mucosa-associated lymphoid tissue (MALT) lymphoma. It is also expressed both in normal lymphoid tissue but doesn't exhibit crossreactivity with other tissues except breast. In non-malignant B-cell follicles the protein is expressed in the cytoplasm of the germinal center B-cells, while in MALT lymphomas with t(1,14)(p22,q32), Bcl 10 expression can be detected both in nucleus and cytoplasm. Applications: Suitable for use in Western Blot, ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:250-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.