Anti-b2-Microglobulin (b2M)

Beta 2-microglobulin is the low molecular weight component of HLA antigens and as such is involved in the presentation of endogenous antigens, activation of T lymphocytes and functional emergence of natural killer cells. It is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning and other conditions leading renal tubular dysfunction. ESTs have been isolated from libraries generated using virtually every tissue in the body. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000, Western Blot: 1:100-1:5000, Immunohistochemistry (Paraffin): 5ug/ml, Immunoprecipitation: 1:2000-1:10,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.