Anti-ASC (PYCARD, PYD and CARD domain containing, ASC, CARD5, MGC10332, TMS1, apoptosis-associated

Alternate Name: PYCARD, PYD and CARD domain containing, ASC, CARD5, MGC10332, TMS1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1 (1-3). Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4). Overexpression of ASC/TMS1 induced DNA fragmentation (4). ASC/TMS1 is expressed in a variety of human and mouse tissues (1, 2) , Domain Information: PYD and CARD domain,, Gene Ontology Terms: caspase activation, caspase activator activity, cytoplasm, induction of apoptosis, negative regulation of cell cycle, protein binding, regulation of apoptosis, signal transduction, , Application(s):, Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 22kD band observed in Hela and Human Kidney lysates (predicted MW of 22kD according to NP_037390.2). Recommended for use at 1-3ug/ml. Staining (1ug/ml) of U937 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A3598-05B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.