Anti-ANGPTL4 (Angiopoietin-related Protein 4, Angiopoietin-like Protein 4, Hepatic Fibrinogen/Angiop

Human Angiopoietin4(Ang4) (1), alternatively named Ang3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structuralmotifs of angiopoietins including the coiledcoileddomain near the aminoterminusand a fibrinogen like domain at the Cterminus. Human Ang 4 cDNA encodes a 503aa precursor protein with a 23aa signal peptide. It shares 45%, 47% and 54% aa sequence identity with human Ang1, human Ang2 and mouse Ang3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang1(97%) and Ang2 (85%), mouse Ang3 is believed to be an ortholog of human Ang4 based on chromosomal localization studies (2). Human Ang4, is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang3 is expressed multiple mouse tissues. Human Ang4 is an agonist that can bind and activate Tie2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2,3). Mouse Ang3 has been reported to be a Tie2 antagonist. It is likely that mouse Ang3, like Ang2, may exert agonist or antagonist activities depending on the cell content(1,3,4). Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.10.4ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.