Anti-alpha-Enolase

alpha-Enolase, also known as enolase 1, is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It is ubiquitously expressed in human tissues, including liver, spleen, kidney, and brain. In cells, alpha-enolase is primarily localized to the cytoplasm, however, an alternatively translated form localizes to the nucleus and lacks glycolytic enzyme activity. alpha-Enolase also functions as a cell surface receptor for plasminogen on pathogens and activated immune cells, as an oxidative stress protein in endothelial cells, and as a chromatin binding partner to facilitate transcription. The ENO1 promoter contains a hypoxia-response element, allowing alpha-enolase to facilitate aerobic glycolysis and contribute to the Warburg effect in tumor cells. alpha-Enolase is overexpressed in multiple tumors, including glioma, neuroblastoma, pancreatic, prostate, and hepatocellular carcinomas. Its role as a plasminogen receptor facilitates extracellular matrix degradation and cancer invasion. alpha-Enolase is an autoantigen in asthma, Hashimoto's encephalopathy, and rheumatoid arthritis, and has been found in the serum of pediatric patients with juvenile idiopathic arthritis. Cayman's alpha-Enolase Polyclonal Antibody can be used for Western blot and ELISA applications. The antibody recognizes alpha-enolase at ~47 kDa from human samples.Synonyms: ENO1, Enolase-1, MBP-1, MPB-1, NNE, Non-Neural Enolase, Phosphopyruvate Hydratase, Plasminogen-Binding Protein. Immunogen: Full length recombinant human alpha-enolase. Formulation: (Request formulation change), 250 µg of protein A-purified polyclonal antibody. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Applications: ELISA, IHC, and WB. UniProt Accession #: P06733.