Anti-Afadin (AF-6, MLLT-4, Myeloid/Lymphoid or mixed-lineage Leukemia Translocated to Ch 4, AFAD, ML

Afadin, also MLLT-4 (Myeloid/Lymphoid or mixed-lineage Leukemia Translocated to Ch 4) and AF6, is a 200-210 kDa nectin and F-actin-binding intracellular protein. It is ubiquitously expressed, and serves as an intermediary that links nectin with the actin cytoskeleton in tight and adherens junctions (AJs). Afadin is involved in the nectin-mediated recruitment of catenins to zonule adherens complexes, and in the recruitment of ZO-1 to the apical side of AJs for the purpose of forming tight junctions. Human Afadin is 1824 amino acids (aa) in length. The protein contains two Ras-association (RA) domains (aa 20-348), a phosphoprotein FHA domain (aa 426?492), a Vav2 Ras-activating "dilute" domain (aa 668-908), one PDZ domain that binds protein C-termini (aa 1007-1093), and three proline-rich regions (aa 1346?1708). There are at least fourteen utilized Ser/Thr phosphorylation sites and two Tyr phosphorylation sites. There are at least seven alternative splice variants are reported for Afadin. There is a 190 kDa isoform that shows a 12 aa insert after Arg1605 coupled to a four aa substitution for aa 1650-1824. Other isoforms contain scattered deletions of one to three aa coupled to major aa changes. Five isoforms share a deletion of aa 393-407, with four of these containing a 24 aa substitution for aa 1605-1824, a 73 aa substitution for aa 1747-1824, an 11 aa substitution for aa 680-1824, and a deletion of aa 1683-1746, respectively. A final isoform possesses a four aa substitution for aa 1650-1824. Over aa 217-392, human Afadin shares 94% aa sequence identity with mouse Afadin. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 3-­15ug/ml, mmunocytochemistry: 1-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.