Anti-Acylation Stimulating Protein (ASP)

Acylation Stimulating Protein (ASP, also known as C3a desArg) is one of the activation fragments formed from the activation of a complement cascade. ASP is produced through a process involving three proteins: C3, factor B and adipsin, which are secreted by adipocytes. Interactions of C3 with factor B and with adipsin result in the production of C3a followed by desargination of the carboxyl terminus to generate ASP (C3a desArg). Human ASP contains 77 amino acids with 6 cysteins involved in disulfide bridges between residues 22-49, 23-56 and 36-57. ASP is a highly cationic molecule containing no carbohydrate. ASP has a primary role in the regulation of lipid metabolism in adipocytes, where it stimulates glucose uptake, increases the activity of diacylglycerol acyltransferase, and inhibits hormone-sensitive lipase activity. In cellular studies, ASP increases fat storage through increased triglyceride synthesis and decreased intracellular lipolysis. In animal models, ASP-deficient mice demonstrate reduced body weight, reduced leptin and reduced adipose tissue mass. ASP deficiency also results in protection against development of obesity. In humans, a number of studies have shown the relationship between ASP, obesity, diabetes and dyslipidemia. It was reported that the concentration of circulating ASP is positively related to body adiposity and decreases after weight loss. Because ASP enhances triglyceride storage, while interfering with ASP production reduces body fat and protects against diet-induced obesity and insulin resistance, reduction of the production of ASP and ASP receptor antagonists represents a potential approach for treating obesity and type 2 diabetes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.