Anti-A20 Zinc Finger Protein, clone 4H16

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-kB gene expression. A20 inhibits NF-kB dependent gene expression by interfering with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IkB kinase pathway. A20 is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues. , Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: Intracellular, Western Blot: 2-4ug/ml, Immunoprecipitation: 1-2ug/ml, Immunohistochemistry: Paraffin , Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, THP1, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.