VEGF-D (human, recombinant)

VEGF-D is a member of the PDGF/VEGF family of growth factors that promotes angiogenesis and lymphangiogenesis. VEGF-D exists as a secreted homodimer composed of a central VEGF homology domain containing the receptor binding region that is flanked by N- and C-terminal propeptides, which are proteolytically cleaved by furin and ADAMTS3 or plasmin to generate the mature, active protein. VEGF-D is produced by macrophages, fibroblasts, smooth muscle cells, and tumor cells and VEGFD expression is dependent on FOS expression and decreased by stimulation with IL-1beta. Binding of VEGF-D to its receptors, VEGFR2 and VEGFR3, which are expressed by endothelial cells, stimulates the survival, migration, and proliferation of endothelial cells and promotes lymphatic and vascular vessel growth and remodeling. VEGF-D induces proliferation and chemotaxis in KS-IMM cancer cells and stimulates growth and capillary-like structure formation in human umbilical vein endothelial cells (HUVECs). Vegfd-/- mice, unlike Vegfc-/- mice, are viable and have a functional lymphatic system, indicating that VEGF-D is dispensable for its development. Tumor VEGF-D levels are increased in patients with epithelial ovarian carcinoma and positively correlated with lymph node metastasis, poor prognosis, and decreased 10-year survival. Cayman's VEGF-D (human, recombinant) protein can be used for cell-based assays applications. This protein consists of 120 amino acids and has a calculated molecular weight of 13.6 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 20 to 22 kDa due to glycosylation.Synonyms: c-Fos-induced Growth Factor, FIGF, Vascular Endothelial Growth Factor D. Purity: >95% as determined by SDS-PAGE.. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant human C-terminal His-tagged VEGF-D expressed in HEK293 cells. Amino Acids: 93-201. MW: 13.6 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: O43915.