Tumor Necrosis Factor Ligand Superfamily Member 13B, active (TNFSF13B), Recombinant, Human

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. {ECO:0000269, PubMed:10973284}., Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. {ECO:0000250}., Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. {ECO:0000269, PubMed:10973284}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 ug/ml. Sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL, Molecular Weight: 17.2kD, PubMed ID: 10e331498, 10e359578, 10e398604, 12867412, 22749832, 12975309, 15057823, 15489334, 10973284, 11853672, 11827482, 11862220, Gene Name: TNFSF13B,BAFF,BLYS,TALL1,TNFSF20,ZTNF4,UNQ401/PRO738, Swiss Prot: Q9Y275, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.