Tumor Necrosis Factor-alpha (TNFa), equine recombinant (reTNF-alpha)

Equine Tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response. Recombinant protein corresponding to equine TNFa, expressed in Pichia pastoris. , Biological Activity:, Determined by measuring the cytolytic effect of recombinant equine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D the ED50 is <0.05ng/ml. Applications: Suitable for use in In vitro applications. Other applications not tested. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.