Tumor Necrosis Factor alpha (TNFa), bovine recombinant (rbTNFa)

Source:, Recombinant protein expressed in Pichia pastoris , Description: Bovine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. Biological Activity: ED50 in the range of 1-10 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D. Endotoxin Level: 0.05EU/ug. Applications: Suitable for use in In Functional assays. Other applications not tested. , Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume with distilled water and store at -70°C. Reconstituted product is stable for 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.