Tumor Necrosis Factor alpha Mutant/Variant, human recombinant (rHuTNF-a-M)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. (www.uniprot.org) Produced in E.coli as a single, non-glycosylated polypeptide chain containing 151 amino acids. The mutant has an amino acid sequence deletion from amino acid 1-7, and the following amino acid substitutes Arg8, Lys9, Arg10 and Phe157 which is proven t