Platelet-Derived Growth Factor subunit B, active (PDGFB), Recombinant, Human

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). {ECO:0000250}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3ng/ml, corresponding to a specific activity of ? 3.3x10e5 IU/mg. Sequence: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT, Molecular Weight: 24.8kD, PubMed ID: 6740330, 4033772, 2991848, 3472769, 3517869, 15461802, 10591208, 15489334, 7659502, 8988177, 6327048, 3456904, 6306471, 6844921, 6329745, 1661670, 1317862, 11331882, 12660034, 18483217, 1396586, 20534510, 23913003, Gene Name: PDGFB,PDGF2,SIS, Swiss Prot: P01127, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.