Interleukin-33 (IL-33), human recombinant (rHuIL-33)

Interleukin 33 (IL-33) is a 32kD proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kD and C-terminal fragment of 18kD by cleavage of caspase-1. Recombinant human IL-33(C-terminal fragment) was expressed in E. coli and purified by using conventional chromatography techniques., , Molecular Weight: 18kD (160 amino acids), Sequences: MSITGISPIT/ EYLASLSTYN/ DQSITFALED/ ESYEIYVEDL/ KKDEKKDKVL/ LSYYESQHPS/ NESGDGVDGK/ MLMVTLSPTK/ DFWLHANNKE/ HSVELHKCEK/ PLPDQAFFVL/ HNMHSNCVSF/ ECKTDPGVFI/ GVKDNHLALI/ KVDSSENLCT/ ENILFKLSET, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.