Interleukin 25 / IL25 / IL17E Protein (Fc tag) (recombinant rat)

Activity: Measured by its binding ability in a functional ELISA.Immobilized mouse IL17BR-His at 10 µg/mL (100 µl/well) can bind rat Fc-IL25. The EC50 of rat Fc-IL25 is 0.12-0.27 µg/mL. Protein Construction: A DNA sequence encoding the rat IL25 (D3ZLB1) (Val17-Ala169) was expressed, fused with Fc region of human IgG1 at the N-terminus. Sequence: Val17-Ala169. Fusion tag: N-Fc Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method Apparent Molecular Mass: 49 kDa. Protein function: Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17-producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)-activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesi