Interleukin 1 alpha, Recombinant, Human, aa113-271, His-Tag

IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. IL-1alpha is a pleiotropic cytokine involved in various immune responses, inflammatory, processes, and hematopoiesis. This cytokine is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Source: Recombinant protein corresponding to aa113-271 from human Interleukin 1 alpha, expressed in E. coli.. Molecular Weight: ~22.4kD (197aa), Endotoxin: ~1EU/1ug (LAL), Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04ng/ml. AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA PFSFLSNVKY NFMRIIKYEF ILNDALNQSI IRANDQYLTA AALHNLDEAV KFDMGAYKSS KDDAKITVIL RISKTQLYVT AQDEDQPVLL KEMPEIPKTI TGSETNLLFF WETHGTKNYF TSVAHPNLFI ATKQDYWVCL AGGPPSITDF QILENQA, Applications: Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.