Fibroblast growth factor 4 (FGF4), human recombinant (rHuFGF4)

Fibroblast growth factor 4 is a member of the FGF family, a group of 7 homologous polypeptides that act upon a variety of cells and produce biological actions that may include cell proliferation, migration and differentiation. FGF4, also known as oncogene products hst1 and KFGF, has strong transforming potential and is a potent angiogenic factor, being expressed in several highly vascularized tumors. Source: Recombinant corresponding to 182aa from human Fibroblast Growth Factor 4 expressed in E. coli. Molecular Weight: ~19.7kD, Endotoxin: <0.1ng/ug , Biological Activity: , ED50 <0.5ng/ml (>2x10e6 units/mg), determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. FGF-4 is a heparin binding growth factor expressed by tumor cells and embryonic cells which has mitogenic activity with fibroblasts and endothelial cells. Applications: Suitable for use in Neutralization. Other applications not tested. Recommeded Dilutions: Optimal dilution to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.