Tankyrase 1 (PARP5A), active human recombinant protein, N-terminal GST tag

TNKS1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1), also known as PARP5A, TANK1, or tanykyrase-1 (GenBank Accession No. NM_003747), a.a. 1000-1328(end), with N-terminal GST-tag, MW=62.5 kDa, expressed in Sf9 cells via a baculovirus expression system. Autoribosylation reaction is conducted in wells coated with various amounts of TNKS1 at 30ºC for 2 hours. Assay buffer: 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2 and 20 ?M ZnCl2. One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.