PKG1 alpha, cGMP dependent, human recombinant, N-terminal His-tag

cGMP dependent Human PKG1alpha (GenBank Accession No. NM_001098512), full length with N-terminal His tag, MW = 80 kDa, expressed in Baculovirus infected Sf9 cell expression system. Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.