IL-10 R alpha, Recombinant, Human, aa22-235, His-Tag

IL-10 R alpha, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immuno suppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Source: Recombinant protein corresponding to aa22-235 from human IL-10 R alpha fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~25.2kD (220aa), Endotoxin: ~1EU/1ug (LAL), Biological Activity: Measured by its ability to inhibit proliferation using MC/9 mouse mast cells. The ED50 for this effect is < 300 ng/ml with Human IL-10. AA Sequence: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSYDLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILGKIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS CVQVKPSVAS RSNKGMWSKEECISLTRQYF TVTNHHHHHH, Applications: Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.