12-Lipoxygenase (platelet-type, mouse recombinant)

12-Lipoxygenase (12-LO) catalyzes the formation of 12-HpETE from arachidonic acid. There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics. Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE. Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.Synonyms: Arachidonate 12-Lipoxygenase, 12-LO, 12-LOX. Purity: >80% estimated by SDS-PAGE. Source: Active recombinant mouse C-terminal His-tagged protein expressed in insect cells. MW: 76 kDa. Formulation: (Request formulation change), 50 mM sodium phosphate pH 7.2, 100 mM sodium chloride, 20 µM FeCl2, and 30% glycerol. UniProt Accession #: P39655.