Notch1dE Lentivirus

Notch1dE Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These lentiviruses express a truncated human Notch1 construct (Notch1dE) in which the entire extracellular domain was deleted. The lentiviruses also contain a puromycin selection marker (Figure 1). Once expressed in a cell line of interest, Notch1dE is cleaved by gamma-secretase, resulting in the constitutively active intracellular domain of Notch (NICD). NICD translocates to the nucleus, binds to the transcription factor CSL (CBF1/RBPJkappa/Suppressor of Hairless/Lag-1) and activates transcription of Notch1-responsive genes. When used in combination with CSL (CBF1/RBP-Jk) Luciferase Reporter Lentiviruses (BPS Bioscience #78746), NICD activates the CSL (CBF1/RBPJkappa/Suppressor of Hairless/Lag-1) responsive elements, inducing luciferase expression.