BMP-2 (Bone Morphogenetic Protein 2) BioAssay(TM) ELISA Kit (Rabbit)

Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF- beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rabbit BMP-2, constituting dimer by two chains of 114aa with the molecular mass of 26kD. Sample Type: Cell culture supernates, serum, bone tissue, tissue. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rabbit BMP-2. Sensitivity: <2pg/ml, Range: 31.2pg/ml-2000pg/ml, Specificity: Recognizes natural and recombinant Rabbit BMP-2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rabbit BMP-2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit BMP-2 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit BMP-2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rabbit BMP-2 antibody, 1x plate, Lyophilized recombinant Rabbit BMP-2 standard, 2x10ng/tube, Biotinylated anti-Rabbit BMP-2 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.