Amyloid Precursor Protein, Human (APP) BioAssay(TM) ELISA Kit

Sensitivity: , <0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times. Specificity: The Human APP BioAssay(TM) ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42. Range: 0.78-50ng/ml , Incubation Time: , 4 hours, Sample Size: 10ul (diluted to a total volume of 100ul), Sample Type: Cell lysates and cerebral spinal fluids, Specifications: Simple: Pre-coated strip-well plates., Liquid stable chromogen and stop reagents., Economical: One 96-well plate, plus reagents for 96 determinations., Reagents are stable for multiple runs. Precision: Intra-assay: <8%, Inter-assay: <9%, Kit Components: *A2275-69L1: Standard, 2x1 vial, Supplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer., A2275-69L2: Standard Diluent Buffer,1x25ml, Supplied as a liquid containing 8mM sodium azide., A2275-69L3: Microtiter Plate, 1x96 wells , A2275-69L4: anti-APP (Biotin), 1x11ml, A2275-69L5: Streptavidin (HRP) (100X), 1x125ul, Supplied as a liquid containing 1.7mM thymol., A2275-69L6: Streptavidin (HRP) Diluent, 1x25ml, Supplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300., A2275-69L7: Wash Buffer (25X), 1x100ml, A2275-69L8: TMB, 1x25ml, A2275-69L9: Stop Solution, 1x25ml, Storage and Stability: Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.