b-Amyloid, aa1-40, Human, BioAssay ELISA Kit

The United States Biological Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen. Sensitivity: <6pg/ml, Range: 7.8-500pg/ml, Kit Components: *A2275-73M1: beta Amyloid 1-40 Standard, 1x1vial, A2275-73M2: Standard Diluent Buffer, 1x60ml, A2275-73M3: Microtiter Plate, 1x96 wells, A2275-73M4: beta Amyloid 1-40, Pab 1x6ml, A2275-73M5: IgG (HRP) (100X), 1x125ul, A2275-73M6: HRP Diluent, 1x25ml, A2275-73M7: Wash Buffer (25X), 1x100ml, A2275-73M8: TMB, 1x25ml, A2275-73M9: Stop Solution, 1x25ml, Storage and Stability: Store *A2275-73M1 powder at 4°C liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.