BRG1 Inhibitor Screening Assay Kit

The BRG1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRG1 binding to its substrate. The kit comes in a convenient AlphaLISA format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged BRG1 bromodomain to perform a total of 384 enzyme reactions. The key to the BRG1 Inhibitor Screening Assay Kit is the specific binding of the BRG1 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRG1 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.