Aldo-Keto Reductase (ARKs) Activity Assay Kit

Detection principle: Aldo-keto reductase (AKRS) is distributed in prokaryotes and eukaryotes, and there are a variety of isoforms. AKRS can catalyze the NADPH-dependent carbonyl reduction reaction to generate the corresponding alcohols. Studies have shown that AKRS are associated with a variety of cancers. The principle of this kit is that the substrate is catalyzed by AKRS, and the chromogenic substance produced by the reaction of the product and the chromogenic agent has a characteristic absorption peak at 450 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of AKRS enzyme in the sample can be calculated. Performance characteristics: Synonyms: ARKs Sample type: serum,plasma,tissue Sensitivity: 0.05 U/L Detection range: 0.05-45.00 U/L Detection method: Colorimetric method Assay type: Enzyme Activity Assay time: 50 min Precision: Average inter-assay CV: 7.1-10%Average intra-assay CV: 1.9-4.8% Other instruments required: Incubator (37?) Other reagents required: Double distilled water, Normal saline (0.9% NaCl) Storage: -20? Valid period: 12 months