Anti-Notch-1(9930111A19RIK, hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOTC

Notch-1 (so named for "notches" in fly wings, also TAN-1) is a 300kD, 2538aa member of the Notch family of type I TM glycoproteins. It is associated with gene activation in both embryo and adult. It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD , disulfide-linked extracellular domain (aa18-1664), and a 120kD membrane-bound segment (aa 1665-2556) that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Human Notch-1 ECD (aa19-526), including the first 13 EGF repeats, shows , 91% aa identity with corresponding regions of mouse and rat Notch-1, and 60% aa identity with human Notch-2 and Notch-3. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Maybe stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.j